Coding
TCepsE
Part:BBa_K2471002
Designed by: Armando Palacios Chaparro Group: iGEM17_Tec-Chihuahua (2017-10-24)
Revision as of 05:31, 24 October 2017 by Armando.Pach (Talk | contribs)
(T7 promoter + RBS) + (aiiA) + (RBS) + (yhjH) + (RBS) + (epsE) + (T1 terminator)
This BioBrick™ contains the necessary genetic circuitry to constitutively express the epsE gene, that when expressed, results in the production of the epsE enzyme. This will cause the disengagement of the flagellum from the motor proteins in the cell membrane, promoting biofilm formation and inhibiting motility.
A chassis with the T7 polymerase gene is needed for the successful expression of this BioBrick™.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2434
Illegal AgeI site found at 137
Illegal AgeI site found at 2184 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |