Generator

Part:BBa_K2381016

Designed by: Wenqi Huang   Group: iGEM17_HZAU-China   (2017-10-23)
Revision as of 23:43, 23 October 2017 by Registry (Talk | contribs)

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light-activated conventional split dCas9 generator

Insert the gRNA sequence behind the PcpcG2, the promoter corresponding to CcaS-CcaR system. The transcription of gRNA will be under the control of light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4470
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2336
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3133


[edit]
Categories
Parameters
None