Reporter

Part:BBa_K2429019:Design

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-23)
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3 Exon eYFP Reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2282
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2282
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2282
    Illegal BamHI site found at 1
    Illegal BamHI site found at 1412
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2282
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2282
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The second HBG intron has a point mutation at bp 654 to match the intron used in a paper that used the HBG intron as part of their reporter.



Source

The human beta globin introns came from the HEK cell genome. The sequences for YFP and BFP were obtained through BLAST, and the part comes from the genome of a jellyfish.

References