Reporter

Part:BBa_K2429018

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-23)
Revision as of 21:18, 23 October 2017 by Registry (Talk | contribs)

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3 Exon mKate-HBG Reporter

This part splits the red fluorescent mKate reporter into two exons, with human beta globin (HBG) intron 2 in between The first mKate exon and an exon that contains a stop codon. HBG intron 1 is placed between the exon with a stop codon and the second mKate exon. We used this split mKate construct as a reporter construct that we used to determine if our dCas13 or Ms2 systems were successful in controlling the inclusion of an exon. We used HBG Introns because it is a standard intron used in reporter constructs

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1762
    Illegal XbaI site found at 1167
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1762
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1762
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1762
    Illegal XbaI site found at 1167
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1762
    Illegal XbaI site found at 1167
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 55


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