![](https://parts.igem.org/images/partbypart/icon_reporter.png)
Reporter
Part:BBa_K2429018
Designed by: Nia Myrie Group: iGEM17_MIT (2017-10-23)
3 Exon mKate-HBG Reporter
This part splits the red fluorescent mKate reporter into two exons, with human beta globin (HBG) intron 2 in between The first mKate exon and an exon that contains a stop codon. HBG intron 1 is placed between the exon with a stop codon and the second mKate exon. We used this split mKate construct as a reporter construct that we used to determine if our dCas13 or Ms2 systems were successful in controlling the inclusion of an exon. We used HBG Introns because it is a standard intron used in reporter constructs
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1762
Illegal XbaI site found at 1167 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1762
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1762
Illegal BamHI site found at 1 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1762
Illegal XbaI site found at 1167 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1762
Illegal XbaI site found at 1167 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 55
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Categories
Parameters
None |