Composite
Part:BBa_K2332003
Designed by: Paola Handal Group: iGEM17_UCL (2017-09-29)
Revision as of 19:05, 23 October 2017 by Paola handal (Talk | contribs)
tRNA-Pyl (pylT)
This composite part encodes tRNA-Pyl (pyIT), a tRNA with the anticodon CUA able to incorporate the photocaged amino acid pyrrolysine (Ne-methyl-L-lysine), into proteins in E. coli. Pyrolysyl-tRNA synthetase first loads the tRNA with pyrrolysine present in the media. The charged pyIT can then incorporate pyrrolysine into proteins where the corresponding sense codon TAG, is present in the mRNA during translation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |