DNA

Part:BBa_K2243009

Designed by: Li Yulong   Group: iGEM17_Peking   (2017-10-23)
Revision as of 18:55, 23 October 2017 by Registry (Talk | contribs)

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phiC31 attL_J23119_phiC31 attR

The constitutive promoter J23119 is between phiC31 RDF specific sites and will be inverted by the phiC31 recombinase and its RDF(or their fusion protein), thus leading to the direction of promoter and change of gene expression. This unit can be used as standard reporter of recombinase phiC31-RDF fusion protein characterization.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 82
    Illegal NheI site found at 105
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None