DNA

Part:BBa_K2243008

Designed by: Chen Hong   Group: iGEM17_Peking   (2017-10-23)
Revision as of 18:52, 23 October 2017 by Registry (Talk | contribs)

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phiC31 attB_J23119_phiC31 attP

The constitutive promoter J23119 is between phiC31 specific sites and will be inverted by the phiC31 recombinase, thus leading to the direction of promoter and change of gene expression. This unit can be used as standard reporter of recombinase phiC31 characterization.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 86
    Illegal NheI site found at 109
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None