![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_K2243007
Designed by: Li Yulong Group: iGEM17_Peking (2017-10-23)
Bxb1 attL_J23119_Bxb1 attR
The constitutive promoter J23119 is between Bxb1 gp35 specific sites and will be inverted by the Bxb1 gp35 recombinase and its RDF bxb1 gp47(or their fusion protein), thus leading to the direction of promoter and change of gene expression. This unit can be used as standard reporter of recombinase bxb1 gp35-gp47 fusion protein characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 96
Illegal NheI site found at 119 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 41
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 61
Illegal BsaI.rc site found at 160
[edit]
Categories
Parameters
None |