Translational_Unit

Part:BBa_K2243003

Designed by: Li Yulong   Group: iGEM17_Peking   (2017-10-23)
Revision as of 16:13, 23 October 2017 by YL18310188100 (Talk | contribs)


D7_Bxb1 gp35

Through the random mutation from iGEM registry RBS, one well-behaved RBS D7 is constructed upstream Bxb1 gp35 coding sequence. The Bxb1 gp35 integrase performed high efficiency of DNA inversion using this construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 192
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 466
    Illegal XhoI site found at 553
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1105
    Illegal NgoMIV site found at 1192
    Illegal AgeI site found at 242
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1300


[edit]
Categories
Parameters
None