Composite

Part:BBa_K2407306

Designed by: Xinyu Chen   Group: iGEM17_Tianjin   (2017-10-12)
Revision as of 15:01, 23 October 2017 by 01is01 (Talk | contribs)


TEF Promoter + vox + Ura3 + Ura3 terminator + vox + β-carotene

Initially, the composite regulates the expression of ura3. When the vika-vox system begin to work, the crtE gene with the remaining composite of β-carotene gene will synthesize β-carotene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2145
    Illegal BglII site found at 3832
    Illegal XhoI site found at 1844
    Illegal XhoI site found at 1976
    Illegal XhoI site found at 2795
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2012
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1125
    Illegal BsaI site found at 4736
    Illegal BsaI site found at 7091
    Illegal BsaI.rc site found at 176
    Illegal BsaI.rc site found at 1572
    Illegal BsaI.rc site found at 7422
    Illegal SapI site found at 4136
    Illegal SapI.rc site found at 972


[edit]
Categories
Parameters
None