Part:BBa_K2235003

Cumate gene switch expressing BFP

This composite part is comprised of a complete inducible system, the cumate system. In particular, there is an expression cassette encoding the reporter gene, BBa_K592100 and there are two additional multiple cloning sites (MCS) flanking the reporter. The repressor of this expression cassette, CymR, is located upstream and it is constitutively expressed. The RNA spinach is an additional reporter protein following CymR aiming to detect its expression upon addition of its cognate fluorophore.

Usage and Biology

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 418
  Illegal XhoI site found at 199
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 101
  

References

1. Choi Y, Morel L, Le Francois T, Bourque D, Bourget L, Groleau D et al. Novel, Versatile, and Tightly Regulated Expression System for Escherichia coli Strains. Applied and Environmental Microbiology. 2010;76(15):5058-5066.