Coding

Part:BBa_K2316001

Designed by: Shaw Kar Ming   Group: iGEM17_NTU_SINGAPORE   (2017-10-23)
Revision as of 11:14, 23 October 2017 by Registry (Talk | contribs)

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∆RuvCIII-2 ∆HNH ∆REC2 Sp-dCas9 Enhanced

The parts is a triple truncation of dCas9 in HNH, Rec2, and RUVCIII2 domain with quick change to enhance dCas9 function. The truncation and quick change was based on the crystal structure of SpCas9. The protein product still functions as dCas9, which has abolished nuclease activity, but with more compact size.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
    Illegal BglII site found at 804
    Illegal BamHI site found at 1622
    Illegal XhoI site found at 2578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1966
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2112
    Illegal BsaI site found at 2774
    Illegal BsaI.rc site found at 1027


[edit]
Categories
Parameters
None