![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K2316000
Designed by: Shaw Kar Ming Group: iGEM17_NTU_SINGAPORE (2017-10-23)
∆RuvCIII-2 ∆HNH ∆REC2 Sp-dCas9
The parts is a triple truncation of dCas9 in HNH, Rec2, and RUVCIII2 domain. The truncation was based on the crystal structure of SpCas9. The protein product still functions as dCas9, which has abolished nuclease activity, but with more compact size.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 251
Illegal BglII site found at 804
Illegal BamHI site found at 1622
Illegal XhoI site found at 2578 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1966
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2112
Illegal BsaI site found at 2774
Illegal BsaI.rc site found at 1027
[edit]
Categories
Parameters
None |