Composite
Part:BBa_K2371010:Design
Designed by: Wang Xiao Group: iGEM17_BGIC-Union (2017-10-23)
sgRNA generator for EML4-ALK variant B 147
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This coding sequence consists of the alternative target sequence of 147B and the scaffold sequence. The target sequence is complementary to one of the sites of the fusion gene EML4-ALK.
Source
DNAworks sequence synthesis