Coding

Part:BBa_K2244003

Designed by: Chen Hong   Group: iGEM17_SSTi-SZGD   (2017-10-18)
Revision as of 05:43, 23 October 2017 by Qiucheng (Talk | contribs)


TorA-opdA


This part is a functional gene that encodes organophosphate hydrolase (OPH) to degrade organophosphate pesticides. In order to export hydrolase to the periplasmic space of E.coli , We fused a TorA signal peptide to the N-terminal of opdA (BBa_K215090) that facilitates the exportation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1123
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 220
    Illegal AgeI site found at 415
    Illegal AgeI site found at 754
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None