Regulatory
Pmini

Part:BBa_K2314831

Designed by: Lei Zhu   Group: iGEM17_OUC-China   (2017-10-22)
Revision as of 00:42, 23 October 2017 by Ykkchen (Talk | contribs)

Pmini is a very short constitutive promoter in yeast, which is only 116bp, but with a good strength.

"Most of native yeast promoters can stretch hundreds of base pairs. Specifically, a single-gene circuit carrying a 1.5kb gene requires an additional 1kb of regulatory DNA (between the promoter and terminator) for appropriate expression, thus increasing the DNA cargo load by over 60%. However, this problem hasn’t been focused widely. With the development of synthetic biology, creating short but strong promoter is more important. Pmini is a short but strong constitutive promoter in yeast. It consists three main elements. Core element determines the shortest length required for transcription and serves as a platform for hybrid promoter technology. This core element scaffold was built on distinct, essential sequences for promoter function—a TATA box with consensus sequence of TATAWAWR24 followed by a transcription start site (TSS) with consensus sequence of A(Arich)5NYAWNN(Arich)6. UAS element contains transcription factor-binding sites (TFBS) and can aid in RNAP stabilization to enhanced transcription rates. The AT-rich spacer containing 30 nucleotides has a better performance.[1] We used Pmini promoter with synthetic terminator Tmini to construct a “Mini system“ by constructing four circuits. We wanted to compare the expression of “Mini system” with that of the common “CYC1 system” which contains Pcyc1 and Tcyc1. "


In short,Pmini is a very short constitutive promoter in yeast, which is only 116bp in length, but with strong expression.


This is the structure of this part.


<img src="T--OUC-China-utrrbs.png

"/> Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part/nominee
//chassis/eukaryote/yeast
//promoter
Parameters
chassisyeast