Composite

Part:BBa_K2332005:Design

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-12)
Revision as of 00:19, 23 October 2017 by Paola handal (Talk | contribs) (→‎Source)


EL222, photosensitive DNA-binding protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 142
    Illegal AgeI site found at 367
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to reverse translate and codon optimize the protein sequence for its usage in E. coli and ensure no illegal sites were present.


Source

Sequence obtained from the reverse translation of EL222 (UniProtKB/Swiss-Prot: [http://www.uniprot.org/uniprot/Q2NB98 Q2NB98.1]) then codon optimized for E. coli. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)

References