Composite

Part:BBa_K2332014

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-16)
Revision as of 00:01, 23 October 2017 by Paola handal (Talk | contribs)


Blue light inducible expression of Intimin'-SpyCatcher (Pblind Intimin'-SpyCatcher)

This biobrick is the fusion of a truncated version of Intimin (159kDa) and SpyCatcher (138 amino acids, 15 kDa) for the cell surface display of SpyCatcher under the control of our blue light inducible promoter (Pblind). To trigger cell adhesion, we decided to make use of the SpyTag and SpyCatcher, two binding partners that form rapid covalent bonds, fused to outer membrane surface proteins. SpyTag (13 amino acids) and SpyCatcher (138 amino acids, 15 kDa) come from CnaB2 (immunoglobulin-llike collagen adhesin domain) of the FbaB protein, found in the invasive strains of S. pyogenes. We decided to fuse SpyTag and SpyCatcher to outer membrane surface proteins in distinct cell populations that would then aggregate once mixed together post blue light induction.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal NgoMIV site found at 1639
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None