Composite

Part:BBa_K2332053:Design

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-21)
Revision as of 23:44, 22 October 2017 by Paola handal (Talk | contribs) (→‎Design Notes)


Blue light inducible expression of GFP-SpyTag (Pblind GFP-SpyTag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 723


Design Notes

We removed the stop codon in GFP and placed the SpyTag sequence after a linker sequence.

Source

https://parts.igem.org/Part:BBa_K1159201 was the source of the SpyTag sequence https://parts.igem.org/Part:BBa_E0040 was for the GFP

References