Coding

Part:BBa_K2332004:Design

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-12)
Revision as of 23:20, 22 October 2017 by Paola handal (Talk | contribs) (→‎References)


EL222, photosensitive DNA-binding protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 81
    Illegal AgeI site found at 306
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Sequence obtained from the reverse translation of EL222 (UniProtKB/Swiss-Prot: [http://www.uniprot.org/uniprot/Q2NB98 Q2NB98.1]) then codon optimized for E. coli. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)

References

Jayaraman P, Devarajan K, Chua T, Zhang H, Gunawan E, Poh C. Blue light-mediated transcriptional activation and repression of gene expression in bacteria. Nucleic Acids Research. 2016;44(14):6994-7005.