Coding

Part:BBa_K2332032:Design

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-19)
Revision as of 22:23, 22 October 2017 by Paola handal (Talk | contribs) (→‎Design Notes)


GFP-mutSpyCatcher (Lys31X, for photocaging)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662


Design Notes

This construct was designed to not contain any other amber stop codon but at position 31 (Lys31X) (Amberless E. coli must be used to see the effects of photocageing spyCatcher). We removed the stop codon in GFP and placed the SpyTag sequence after a linker sequence and included a HisTag for protein purification.

Source

SpyCatcher: BBa_K1159200 GFPmut 3b: Part:BBa_E0040

References