Coding

Part:BBa_K2332031:Design

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-19)
Revision as of 22:07, 22 October 2017 by Paola handal (Talk | contribs) (→‎Design Notes)


GFP-SpyCatcher


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662


Design Notes

We removed the stop codon in GFP and placed the SpyTag sequence after a linker sequence and included a HisTag for protein purification.

Source

Intimin protein sequence obtained from: http://www.uniprot.org/uniprot/P43261#sequences And reverse translated using: http://www.bioinformatics.org/sms2/rev_trans.html Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001 SpyCatcher: BBa_K1159200 GFPmut 3b: Part:BBa_E0040

References