Coding

Part:BBa_K2332010

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-16)
Revision as of 21:26, 22 October 2017 by Paola handal (Talk | contribs)


Intimin'-SpyTag

This biobrick is the fusion of a truncated version of Intimin (159kDa) and SpyTag (13 amino acids) for the cell surface display of SpyTag. To trigger cell adhesion, we decided to make use of the SpyTag and SpyCatcher, two binding partners that form rapid covalent bonds fused to outer membrane surface proteins. SpyTag (13 amino acids) and SpyCatcher (138 amino acids, 15 kDa) come from CnaB2 (immunoglobulin-llike collagen adhesin domain) of the FbaB protein, found in the invasive strains of S. pyogenes. We decided to fuse SpyTag and SpyCatcher to outer membrane surface proteins in distinct cell populations that would then aggregate once mixed together. Intimin'-SpyTag and Intimin'-SpyCatcher will be placed under the control of our blue light inducible promoter Pblind to guide cell structure formation using blue light.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 801
    Illegal NgoMIV site found at 1542
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None