Device

Part:BBa_K2463000:Design

Designed by: Jack Lawrence, Balint Vidos   Group: iGEM17_Warwick   (2017-10-22)
Revision as of 19:46, 22 October 2017 by Psychosahedron (Talk | contribs) (Reformat #2)


Biofilm and Motility Regulation Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1806
    Illegal BamHI site found at 32
    Illegal BamHI site found at 1190
    Illegal XhoI site found at 1768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
    Illegal NgoMIV site found at 595
    Illegal AgeI site found at 931
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 105


Design Notes

Lacks a degrading tag fused within yhjH gene due to unpredictable interactions.


Source

Source

Uniprot entries for important proteins:

PLeD Source: [http://www.uniprot.org/uniprot/Q9A5I5 uniprot.org/uniprot/Q9A5I5]
yhjH Source: [http://www.uniprot.org/uniprot/P37646 uniprot.org/uniprot/P37646]

iGEM registry entries regarding parts:

BBa_R0010 - LacI
BBa_B0034 - Ribosome binding site
BBa_K2012002 - PLeD gene (synthesises c-di-GMP)
BBa_C0072 - Mnt repressor
BBa_B1006 - Terminator
BBa_R0073 - Mnt promoter
BBa_K861090 - Yhjh (degrades c-di-GMP)
BBa_B1003 - Second Terminator

References