Composite

Part:BBa_K2379005:Design

Designed by: Ramit B   Group: iGEM17_SVCE_CHENNAI   (2017-10-01)
Revision as of 19:18, 22 October 2017 by Aditi (Talk | contribs) (→‎Design Notes)

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RNA thermometer (FourU) with GFP under the regulation of pLAC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 765


Design Notes

The circuit is under the regulation of the pLAC promoter. When inserted in a high copy number plasmid pLAC behaves as a constitutive promoter and hence does not require IPTG induction. However, if inserted in a low copy number plasmid, the system will have to be induced with IPTG as it would then behave like an inducible promoter. GFP, in this case, will be produced only in the presence of IPTG and at 37 degree Celsius.

Source

iGEM Registry

References