Device
Part:BBa_K2463000:Design
Designed by: Jack Lawrence, Balint Vidos Group: iGEM17_Warwick (2017-10-22)
Biofilm and Motility Regulation Device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1806
Illegal BamHI site found at 32
Illegal BamHI site found at 1190
Illegal XhoI site found at 1768 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 448
Illegal NgoMIV site found at 595
Illegal AgeI site found at 931 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 105
Design Notes
Lacks a degrading tag fused within yhjH gene due to unpredictable interactions.
Source
PLeD Source: www.uniprot.org/uniprot/Q9A5I5 yhjH Source: www.uniprot.org/uniprot/P37646
iGEM registry entries regarding parts: BBa_R0010 -lac BBa_B0034 -rbs BBa_K2012002 -PLeD BBa_B0034 -rbs BBa_C0072 -mnt repressor BBa_B1006 -Terminator BBa_R0073 -mnt promoter BBa_K861090 -Yhjh BBa_B1003 -Second Terminator