Coding

Part:BBa_K2203000

Designed by: Lena Bruhin   Group: iGEM17_EPFL   (2017-10-22)
Revision as of 18:26, 22 October 2017 by Lenabruhin (Talk | contribs)


gamS protein GamS is a protein that protects linear DNA templates from degradation in a cell- free environment. It is used to enhance cell-free protein synthesis by inhibiting exonuclease activity.

This part has a demonstrated positive effect on protein synthesis in a cell-free chassis. GamS can be added to a cell-free reaction system in several ways: By adding it in its synthesized protein form, or by adding simply its coding sequence to the cell-free reaction, or (in the case of a self-made cell lysate) by making the lysate from cells which were transformed with the GamS-plasmid. For the first and last example, see experimental results below.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 298
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part/nominee
Parameters
None