Coding

Part:BBa_K2272002

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-21)
Revision as of 18:03, 22 October 2017 by Ew8sk (Talk | contribs)


HAO (hydroxylamine oxidoreductase)

This coding sequence in Nitrosomonas europaea, a nitrifying bacterium, codes for hydroxylamine oxidoreductase, which catalyzes the oxidation of hydroxylamine to nitrite. The P. denitrificans TAT signal peptide coding sequences are embedded in the HAO coding sequence to direct export the hydroxylamine oxidoreductase to the periplasm of the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 339
    Illegal XhoI site found at 1903
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 355
    Illegal NgoMIV site found at 832
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 2604
    Illegal NgoMIV site found at 2994
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 259
    Illegal BsaI.rc site found at 307
    Illegal BsaI.rc site found at 874
    Illegal BsaI.rc site found at 3034


[edit]
Categories
Parameters
None