Composite

Part:BBa_K2505001

Designed by: Hazuki Hasegawa   Group: iGEM17_TokyoTech   (2017-10-16)
Revision as of 17:49, 22 October 2017 by TakumaY (Talk | contribs)


pBad/araC-rbs-ahk4

This part produces AHK4, a receptor of iP(isopentenyl adenine: kind of cytokinin). This part was introduced to E.coli(KMI002) and sensed iP synthesized by human cell. The gene(ahk4) is derived from A.thaliana and optimized for E.coli codon in reference to the codon usage.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1294
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI site found at 1542
    Illegal SapI.rc site found at 3177


[edit]
Categories
Parameters
None