RNA

Part:BBa_K2217001

Designed by: Mohammad Tarek Mansour   Group: iGEM17_AFCM-Egypt   (2017-09-22)
Revision as of 17:42, 22 October 2017 by MTarek (Talk | contribs)


Circular RNA hsa-circ-000064 RNA sequence was retrieved from circ-Interactome database Regulatory circular RNA hsa-circ-0000064 used in Competing endogenous RNA ceRNA circuit, We used this part as a competing endogenuous RNA to regulate Hepatocellular-Carcinoma associated miRNA mir-1825.


Circular RNA Model

Minimum free energy prediction using RNAFOLD generated an optimal secondary structure in dot-bracket notation from a centroid structure of 0.00 kcal/mol minimum free energy Structure

Circular RNA (2).png

to 1.78 kcal/mol minimum free energy Structure

Circular RNA.png

More Information about circular design of this part can be found at [http://2017.igem.org/Modeling.html# Egypt-AFCM Modeling]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 128
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part
Parameters
None