Coding

Part:BBa_K648013

Designed by: Jim Rose   Group: iGEM11_Penn_State   (2011-07-04)
Revision as of 17:18, 22 October 2017 by CamilloMoschner (Talk | contribs)


GFP with Standard 25 Prefix/Suffix

This is the standard GFP protein reporter (E0040) cloned with the prefix/suffix required for standard 25 assembly. It can easily be used to create fusion proteins through this method.


Additional Tag

UCL iGEM 2017 used this BioBrick for its designs and noticed that this BioBrick actually contains the GFP protein reporter (E0040) fused to a FLAG epitope tag, followed by an enterokinase cleavage site. This is important if cell surface expression of this BioBrick is wished. Addition of enterokinase (also called enteropeptidase) to the solution allows easy testing of protein localisation (inside or outside of the membrane).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 641


[edit]
Categories
//cds/reporter/gfp
//function/reporter/fluorescence
Parameters
colorGreen