Part:BBa_K2273034

Part Information
BioBrick Nr.	BBa_K2273034
RFC standard	RFC 25
Requirement	pSB1C3
Original Biobrick Part	BBa_J06504: mCherry
Submitted by	[http://2017.igem.org/Team:TU_Dresden TU Dresden]

Brief introduction in Fluorescent Proteins

 

Fluorescent proteins are small proteins with β-barrel-fold topology. They are useful for tracking global expression of target genes and localizations of these genes inside/outside cells. The unique chromophore in each fluorescent protein, originates from three intrinsic amino acids, at positions 65–67. The chromophore is tightly enclosed inside the protein and its formation does not require any cofactors or enzymes but only molecular oxygen. The rigidity of the β-barrel protects the chromophore from the environment and from radiationless decay. It also restricts chromophore flexibility as the correct folding of the protein is required for the chromophore formation. Proper orientation of the amino acids is necessary for chromophore maturation as it catalyzes chromophore synthesis.

Overview of mCherry

 

mCherry is a red fluorescent protein that has an excitation peak 585 nm and a peak emission at 615 nm.

mCherry expression in Bacillus subtilis

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]