Coding

Part:BBa_K2371003:Design

Designed by: Wang Xiao   Group: iGEM17_BGIC-Union   (2017-10-22)
Revision as of 11:29, 22 October 2017 by Joyce324 (Talk | contribs) (→‎Design Notes)


C-T7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 973
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The first two Biobricks are the two split T7 polymerase sequence we adopted. We provide them for other team to leverage them through other methods. We choose the split site suggested by the team of Tiyun Han, Quan Chen and Haiyan Liu.( Tiyun,H.et al.,2016.Engineered photoactivatable genetic switches based on the bacterium phage T7 RNA polymerase.ACS Synthetic Biology,http://pubs.acs.org on October 31, 2016.)

Source

T7 polymerase from bacteria BL21(DE3)

References