Terminator

Part:BBa_K2407109:Design

Designed by: Yan Zheng   Group: iGEM17_Tianjin   (2017-10-17)
Revision as of 11:24, 22 October 2017 by Tjuyoung (Talk | contribs) (→‎References)

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a terminator used in yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is modified for use in yeast as the terminator of HO. This gene encodes a subunit of the cytochrome bc1 complex, which plays an important role in the mitochondrial respiratory chain by transferring electrons from the Rieske iron-sulfur protein to cytochrome c.

Source

We use PCR to amplify the part from a PRS-415 CAS9 plasmid.

References

Cytochrome c1 in ductal carcinoma in situ of breast associated with proliferation and comedo necrosis. Chishiki M, et al. Cancer Sci, 2017 Jul. PMID 28394473. CYC1 Predicts Poor Prognosis in Patients with Breast Cancer. Han Y, et al. Dis Markers, 2016. PMID 27239088.