Part:BBa_K2232012
P43_OF4-nhaC_rrnBT1
The gene nhaC from the Bacillus pseudofirmus OF4 encodes the Na+/H+ antiporter, which regulates cytoplasmic pH value by coupling net H+ uptake with Na+ extrusion. NhaC, the first demonstrated alkaliphile Na+/H+ antiporter, as part of a complement of antiporters in the wild-type alkaliphile, apparently plays a role over a wide range of pH values. Its most apparent role was observed at pH 7.5 in media containing low Na+ concentrations, where a pronounced defect in growth was observed. Although pH homeostasis is not a major challenge at pH 7.5, the alkaliphile must nonetheless extrude Na+ that enters in symport with solutes, including the growth substrate malate in the media used here. At pH 10.5, NhaC also plays an apparent role at low Na+ concentrations;at the alkaline pH, it contributes to pH homeostasis and may also contribute to the capacity of the cell to lower the cytoplasmic Na+ concentration optimally.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 527
- 1000COMPATIBLE WITH RFC[1000]
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