Composite

Part:BBa_K2325103

Designed by: Xiaojia Liu   Group: iGEM17_SCUT-FSE-CHINA   (2017-10-16)
Revision as of 09:01, 22 October 2017 by KahiuLau (Talk | contribs)


An expression device for fusion protein of Formamidase and Phosphite dehydrogenase

The BBa_K2325001 harbors a coding sequence of and formamidase(FOR) and phosphite dehydrogenase (PTDH) with a linker between them, which can simultaneously catalyzes the conversion of formamide to ammonia and phosphite to phosphate, respectively. The device also contains GST tag for increasing the solubility of exogenous protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 821
    Illegal NgoMIV site found at 1308
    Illegal NgoMIV site found at 1503
    Illegal NgoMIV site found at 1650
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 159


[edit]
Categories
Parameters
None