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Part:BBa_K2407106:Design

Designed by: Yan Zheng   Group: iGEM17_Tianjin   (2017-10-17)
Revision as of 08:52, 22 October 2017 by Tjuyoung (Talk | contribs) (→‎Source)


a constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 840


Design Notes

It is a constitutive promoter. It is translation of translational elongation factor EF-1 alpha. When it is the GTP-bound active form, binds to and delivers aminoacylated tRNA to the A-site of ribosomes for elongation of nascent polypeptides.It also associates with vacuolar Rho1p GTPase. TEF1 may also have a role in tRNA re-export from the nucleus. TEF1 has a paralog, TEF2, that arose from the whole genome duplication.

Source

We use PCR to amplify the part from a plasmid CAN-A which contains a half part of gene CAN and has TEF1 as its promoter.

References