Part:BBa_K2462006

Promoter for Bacillus megaterium+RBS

This part is a combination of promoter and RBS. We got the sequence of RBS (BBa_B0030) from its part page , and combined it to the promoter got from the plasmid PHIS1525. This plasmid can be used in Bacillus magaterium for the expression of foreign proteins. Then the whole part has been synthesized by AuGCT. Bacillus magaterium is a kind of potential genetic engineering host, which is now frequently used in industry and academic fields. It has lots of advantages over other host as following: 1. It is able to yield exceptionally high-level foreign protein. 2. Its heredity is stable 3.The foreign proteins can be secreted out of cell, in that case, we just need to collect the supernatant rather than lyse the cell by sonication. In a word, it can be easily operated. 4 Entotoxin free for bacillus magaterium, which is eligible for biosafety requirement. However, there are few iGEM teams using these strain as their host, so we are going to be one of the pioneers and we try to find several types of promoter, RBS and terminator and plan to make combinations between them. Our final purpose is to find the most effective way for protein expression in Bacillus magaterium for further uses.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]