Coding

Part:BBa_K2450101

Designed by: Zoe Ford   Group: iGEM17_Oxford   (2017-09-28)
Revision as of 15:45, 21 October 2017 by Tinyref (Talk | contribs) (→‎Usage and Biology)


TEV protease tagged with mCherry

A non-self-cleaving TEV protease sequence with an N terminal mCherry tag and a C terminal His tag. The fluorophore tag allows for relative quantification of expression. The His tag allows for purification using a nickel column.

Usage and Biology

TEV protease is a well-characterised specific protease from the Tobacco Etch Virus, with a cleavage sequence of Glu-Asn-Leu-Tyr-Phe-Gln-(CUT)-Gly.

This is a non-self-cleaving protease as we wanted a constant level of TEV protease produced.

We have used TEV protease as both a substitute for cruzipain, another specific protease, and as a signal amplifier after simulated cruzipain cleavage.

This part does not require any other parts to be functional.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1444
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None