Composite
Part:BBa_K2332053
Designed by: Paola Handal Group: iGEM17_UCL (2017-10-21)
Blue light inducible expression of GFP-SpyTag (Pblind GFP-SpyTag)
We designed our GFP-SpyTag Biobrick to test bacterial cell adhesion through fluorescence microscopy. This part produces the GFP-SpyTag fusion protein, which we experimentally used in combination with our Intimin-SpyCatcher variants. To use the GFP-Spytag, one needs to lyse recombinant E. coli cells and purify the protein extract in order to visualize its localization on the cell surface of cells expressing intimin'-SpyCatcher variants.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 723
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Categories
Parameters
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