Composite

Part:BBa_K2332053

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-21)
Revision as of 15:33, 21 October 2017 by Registry (Talk | contribs)

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Blue light inducible expression of GFP-SpyTag (Pblind GFP-SpyTag)

We designed our GFP-SpyTag Biobrick to test bacterial cell adhesion through fluorescence microscopy. This part produces the GFP-SpyTag fusion protein, which we experimentally used in combination with our Intimin-SpyCatcher variants. To use the GFP-Spytag, one needs to lyse recombinant E. coli cells and purify the protein extract in order to visualize its localization on the cell surface of cells expressing intimin'-SpyCatcher variants.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 723


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