Composite
Part:BBa_K2332052:Design
Designed by: Paola Handal Group: iGEM17_UCL (2017-10-21)
Intimin'-GFP-SpyTag (constitutive)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 2826
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 2826 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 2826
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 2826
Illegal NgoMIV site found at 862
Illegal NgoMIV site found at 1603 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2697
Design Notes
Removal of ATG from GFP for a more efficient expression of this fusion protein
Source
Intimin protein sequence obtained from: http://www.uniprot.org/uniprot/P43261#sequences And reverse translated using: http://www.bioinformatics.org/sms2/rev_trans.html Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001 SpyTag: BBa_K1159201 GFPmut 3b: Part:BBa_E0040