Composite

Part:BBa_K2332050

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-21)
Revision as of 15:30, 21 October 2017 by Registry (Talk | contribs)

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Intimin'-SpyTag (constitutive)

This biobrick is for the constitutive expression of Intimin'-SpyTag as part of or bacterial cell aggregation system. This biobrick is the fusion of a truncated version of Intimin and SpyTag for the cell surface display of SpyTag under the control of of a constitutive promoter. When cells expressing this construct are mixed with cells expressing Intimin'-SpyCatcher aggregation is expected to occur. SpyTag (13 amino acids) and SpyCatcher (138 amino acids, 15 kDa) are binding partners that originate from CnaB2 (immunoglobulin-llike collagen adhesin domain) of the FbaB protein, found in the invasive strains of S. pyogenes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 862
    Illegal NgoMIV site found at 1603
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None