Coding

Part:BBa_K2450201:Design

Designed by: Zoe Ford   Group: iGEM17_Oxford   (2017-09-27)
Revision as of 14:37, 21 October 2017 by Tinyref (Talk | contribs)


TetR with TEV cleavage site and CFP tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to decide on an appropriate location for the TEV cleavage site in TetR. We examined the crystal structure of TetR and identified a linker between the DNA binding and dimerization domains.

Source

TetR from BBa_K106669 TEV cleavage sequence from Merck CFP from AddGene

References