Coding
Part:BBa_K2450201:Design
Designed by: Zoe Ford Group: iGEM17_Oxford (2017-09-27)
TetR with TEV cleavage site and CFP tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to decide on an appropriate location for the TEV cleavage site in TetR. We examined the crystal structure of TetR and identified a linker between the DNA binding and dimerization domains.
Source
TetR from BBa_K106669 TEV cleavage sequence from Merck CFP from AddGene