Reporter

Part:BBa_K2450501

Designed by: Zoe Ford   Group: iGEM17_Oxford   (2017-10-17)
Revision as of 14:03, 21 October 2017 by ArthurN (Talk | contribs)


SpyCatcher sfGFP quencher

SpyCatcher, sfGFP, TEV cleavable linker, reach quencher


Usage and Biology

This part was designed to be targeted to the outer membrane. It has a TorA leader sequence which targets it to the periplasm through the Tat translocase system. This translocates folded proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 397
    Illegal SapI.rc site found at 1291


[edit]
Categories
Parameters
None