DNA

Part:BBa_K2350015:Design

Designed by: JO-NING HUNG   Group: iGEM17_NYMU-Taipei   (2017-10-21)
Revision as of 10:12, 21 October 2017 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


3NSII-ORI-5NSII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1866
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2428
    Illegal BamHI site found at 2725
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2646
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2328


Design Notes

To insert 3NSII-ORI-5NSII with EcoR1 and Pst1 cutting sites, we use site-directed mutagenesis to remove Pst1 cutting sites in both 5NSII and 3NSII nucleotide sequence.



Source

pBR322 and Synechoccocus elongatus PCC7942 genomic DNA

References