Composite

Part:BBa_K2311000:Design

Designed by: Wenwen Lin   Group: iGEM17_SiCAU-China   (2017-09-30)
Revision as of 09:15, 21 October 2017 by Lww (Talk | contribs) (→‎Design Notes)


Positive feedback loop (LuxI with LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2315


Design Notes

Source

This part has a positive feedback loop, so the expression of GFP can enhance quickly. In General, a positive feedback loop can be triggered by a low input such as PCR. This positive feedback loop will be opened by background expression of pLuxR in E.coli. But different inphttps://parts.igem.org/Promotersut lead to different opening time.

References