Translational_Unit

Part:BBa_K2230024

Designed by: Yi-Lun Huang   Group: iGEM17_Mingdao   (2017-10-20)
Revision as of 00:14, 21 October 2017 by Registry (Talk | contribs)

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RBS-ptsG-TT/pSB1C3

Salmonella typhimurium LT2 has two glucose-specific transporter systems, PTS system and sodium/glucose cotransporter. PTS system contains two subunits IIA encoded by crr and IIBC by ptsG which are assembled to a high-affinity active transporter. The other is a Na+/glucose cotransporter encoded by STM1128 that contributes to facilitated transport with lower glucose affinity. Based on our research, the glucose transporter of Salmonella has a lower Km compared to human small intestine, Staphylococcus and E. coli, indicating a higher efficiency for glucose uptake. In our project, we created the glucose transporter device and genetically engineer microbes with these two systems.

Cloning: The RBS-ptsG part (BBa_K2230022) was assembled with Double Terminator/pSB1C3 (BBa_B0015)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 801
    Illegal NgoMIV site found at 1224
    Illegal AgeI site found at 508
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None