Coding
Part:BBa_K2374003:Design
Designed by: Qian Zeng Group: iGEM17_Tongji_China (2017-10-20)
ple (Tyrosine 3-monooxygenase, TH) -> (fruit fly)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1157
Illegal XhoI site found at 289 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 535
Illegal BsaI site found at 1501
Illegal BsaI.rc site found at 253
Illegal BsaI.rc site found at 1019
Illegal BsaI.rc site found at 1168
Design Notes
site direct mutangenesis: Pst I (647) CTGCAG->CTGCTG This part Pale CDS is cloned from cDNA, and because of its multi-segment repeats, it is hard to cloned from cDNA, so we recommend that you obtain directly from the constructed plasmid, or synthesize it directly.
Source
Drosophila melanogaster chromosome 3L [NT_037436.4] (NCBI)