Coding

Part:BBa_K2374003:Design

Designed by: Qian Zeng   Group: iGEM17_Tongji_China   (2017-10-20)
Revision as of 00:04, 21 October 2017 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


ple (Tyrosine 3-monooxygenase, TH) -> (fruit fly)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1157
    Illegal XhoI site found at 289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 535
    Illegal BsaI site found at 1501
    Illegal BsaI.rc site found at 253
    Illegal BsaI.rc site found at 1019
    Illegal BsaI.rc site found at 1168


Design Notes

site direct mutangenesis: Pst I (647) CTGCAG->CTGCTG This part Pale CDS is cloned from cDNA, and because of its multi-segment repeats, it is hard to cloned from cDNA, so we recommend that you obtain directly from the constructed plasmid, or synthesize it directly.


Source

Drosophila melanogaster chromosome 3L [NT_037436.4] (NCBI)


References