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Composite
Part:BBa_K2332041
Designed by: Paola Handal Group: iGEM17_UCL (2017-10-20)
Blue light inducible expression of GFP-SpyCatcher (Pblind GFP-SpyCatcher)
This construct is the fusion of GFP with SpyCatcher. This construct allows to directly observe through fluorescence microscopy its localization, and most importantly its co-localization with SpyTag variants. Particularly for our project, it allows us to quantify its binding onto cell surface displayed Intimin'-SpyTag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 741
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Categories
Parameters
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