Coding

Part:BBa_K2455001:Design

Designed by: Jon Fugl   Group: iGEM17_UCopenhagen   (2017-10-20)
Revision as of 13:34, 20 October 2017 by Registry (Talk | contribs)

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His-tagged TrpE gene from E. coli MG1655


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 965
    Illegal BglII site found at 1026
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In addition to having a M293T mutation the TrpE sequence contains a his-tag in order to allow for detection of expression through the use of Western Blotting.


Source

The sequence originated from the MG1655 E. coli strain but carries a M293T point mutation in order to avoid negative feedback inhibition by L-tryptophan (source - Gu et al has further link), in addition a hexa his-tag has been added.

References